distribution of sefa gene among salmonella enteritidis isolates from poultry sources and potential as diagnostic and epidemiological tools
نویسندگان
چکیده
this study was conducted to detect the presence ofseja gene among salmonella enteritidis isolates from poultry sources by polymerase chain reaction (pf'r) and evaluate its potential as diagnostic and epidemiological tools. thirty salmonella isolates from poultry sources: broilers. broiler breeders, layers, hatcheries, and poultry abattoirs were investigated. upper and forward primers were constructed based on the published sequence of the seja gene that encodes the sef14 fimbrial subunit (fimbrin). the size of target product was 526 bp. to confirm the specificity, the pcr products were digested with bam hi restriction enzyme that divides the product to two segments of 186 and 340 bp. the pcr reaction was set up as described in the previous literature. all salmonella enteritidis isolates showed the presence of526 bpproduct. noneofisolates belongingtoserogroups band c were positive for the 526 bp fragment. the restriction enzyme bamhl divided each 526 bp product into two fragments of 186 and 340 bp. this pattern was demonstrated for all salmonella enteritidis isolates. the results of the present study showed that the seja gene carries a high potential to be used as a diagnostic and an epidemiological 1001 for salmonella enteritidis
منابع مشابه
فراوانی ژن sefA در جدایه های سالمونلا آنتریدیس طیور و پتانسیل آن به عنوان یک شاخص تشخیصیو اپیدمیولوژیک
This study was conducted to detect the presence ofseJA gene among Salmonella Enteritidis isolates from poultry sources by polymerase chain reaction (Pf'R) and evaluate its potential as diagnostic and epidemiological tools. Thirty Salmonella isolates from poultry sources: broilers. broiler breeders, layers, hatcheries, and poultry abattoirs were investigated. Upper and forward primers were const...
متن کاملCharacterization of Salmonella isolates from poultry sources in Iran
BACKGROUND: Salmonellosis is one of the most importantzoonotic diseases throughout the world. OBJECTIVES: Thepurpose of this study was to characterize a large collection ofSalmonella isolates from different poultry sources in Iran.METHODS: A total of 123 Salmonella isolates from differentpoultry sources were subjected to drug susceptibility test,hemolysin production, motility test, and plasmid ...
متن کاملPhenotypic and genotypic studies of extended spectrum beta-lactamase (ESBL) resistance among Salmonella isolates from poultry sources in Iran
BACKGROUND: Poultry and poultry products are among the major sources of Salmonella infections for humans. Increasing occurrence of antimicrobial resistance among Salmonellae has become a serious public health concern. The detection of extended spectrum b-lactamase (ESBL) producers among Salmonella spp. has increased in recent years. OBJECTIVES: The purpose of this study was to investigate the a...
متن کاملOccurrence of multidrug-resistant Salmonella enterica serovar Enteritidis isolates from poultry in Iran
Salmonella enterica is recognized as one of the major food-borne pathogens with more than 2,500 serotypesworldwide. The present study addresses antimicrobial resistance of Salmonella enterica serovar Enteritidisisolates in Iran. A collection of 151 Salmonella spp. isolates collected from poultry were serotyped toidentify Salmonella Enteritidis. Sixty-one Salmonella Enteritidis were subsequently...
متن کاملفراوانی ژن sefa در جدایه های سالمونلا آنتریدیس طیور و پتانسیل آن به عنوان یک شاخص تشخیصیو اپیدمیولوژیک
this study was conducted to detect the presence ofseja gene among salmonella enteritidis isolates from poultry sources by polymerase chain reaction (pf'r) and evaluate its potential as diagnostic and epidemiological tools. thirty salmonella isolates from poultry sources: broilers. broiler breeders, layers, hatcheries, and poultry abattoirs were investigated. upper and forward primers were const...
متن کاملcharacterization of salmonella isolates from poultry sources in iran
background: salmonellosis is one of the most importantzoonotic diseases throughout the world. objectives: thepurpose of this study was to characterize a large collection ofsalmonella isolates from different poultry sources in iran.methods: a total of 123 salmonella isolates from differentpoultry sources were subjected to drug susceptibility test,hemolysin production, motility test, and plasmid ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
تحقیقات دامپزشکیجلد ۶۳، شماره ۳، صفحات ۲۲۹-۲۳۴
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023